Evaluation of Periodontal Condition and Subgingival Microbiota in Adolescents Orthodontically Treated With Fixed Braces
Name: Caroline Lopes Binda
Type: MSc dissertation
Publication date: 21/01/2019
Advisor:
Name |
Role |
|---|---|
| Selva Maria Gonçalves Guerra | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Alfredo Carlos Rodrigues Feitosa | Co advisor * |
| Lorena da Rós Gonçalves | External Examiner * |
| Maria Christina Thomé Pacheco | Internal Examiner * |
| Selva Maria Gonçalves Guerra | Advisor * |
Summary: Introduction: Plaque retention around orthodontic brackets may change subgingival microbiota composition and lead to gingival inflammation and, subsequently, to destruction of supporting periodontal tissues. Objective: To evaluate and compare the periodontal health and subgingival microbial plaque composition after orthodontic treatment. Materials and Methods: 23 participants aged between 10 and 19 years who had finished orthodontic treatment were selected by means of a non-probabilistic sampling for convenience method. Subgingival plaque samples were obtained from six index teeth (16, 11, 26, 36, 31 and 46) upon orthodontic brackets removal (T1) and after 6 months (T2), and analyzed by the Checkerboard DNA-DNA hybridization technique; in addition, the participants were submitted to clinical examination in terms of visible plaque index, gingival bleeding index, probing depth and bleeding on probing. Results: A significant reduction of periodontal clinical parameters was observed six months after brackets removal. Also at T2, a significant increase of mean counts and proportions of the bacteria C. showae, F. periodonticum, S. sanguinis, S. gordonii, C. gingivalis, A. odontolyticus I, A. naeslundii I was observed (p<.05), as well as a significant reduction of F. nucleatum (sp. vincentii), E. saburreum, S. anginosus e P. melaninogenica (p<.05), with higher frequency for bacteria from the orange complex (T1: 28.28%; T2: 27.88%). Conclusion: Periodontal health is negatively influenced by the use of fixed orthodontic appliances; however, clinical parameters tend to return to normal values after brackets removal as well as the concentration of periodontopathogenic bacteria present in subgingival plaque.
