ANALYSIS OF THE TYPE I COLLAGEN PROFILE IN SQUAMOUS CELL CARCINOMA ORAL AND THEIR PRECURSOR LESIONS
Name: Renata Escapini Fanchiotti Brandão Amorim
Type: MSc dissertation
Publication date: 09/03/2017
Advisor:
Name |
Role |
|---|---|
| Leticia Nogueira da Gama de Souza | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Danielle Resende Camisasca Barroso | Internal Examiner * |
| Leticia Nogueira da Gama de Souza | Advisor * |
| Liliana Aparecida Pimenta de Barros | Co advisor * |
| Marcos da Silva Pacheco | External Examiner * |
Summary: Squamous cell carcinoma (SCC) is considered the most common cancer of the mouth, and is still associated with high mortality rates. In addition to the evaluation of the clinical aspects, it is important to carry out a more accurate analysis of the molecular profile for a better understanding of its biological behavior. Type I collagen is one of the main components of the extracellular matrix (ECM) and has been associated with the tumorigenesis process due to its participation in events such as angiogenesis and metastasis. The present study carried out an immunohistochemical analysis of collagen type I profile in lesions diagnosed by the Service of Oral Pathological Anatomy of the Dentistry Course/UFES. The groups evaluated were: 10 cases of low risk lesions; 10 cases of high risk of malignization lesions and 30 cases of SCC. Furthermore, possible associations between immunostaining and patient clinical profile were established. Our findings showed that men were the most affected gender in high risk lesions (60%) and SCC (86.6%), while women represented a majority in low risk lesions (80%). In relation to sites, jugal mucosa was the most frequent in low risk lesions (50%), and tongue in high risk cases (60%) and SCC (43.3%). Regard type I collagen profile, in SCC the fibers were mostly moderately irregular (++) and with a strong staining. In low risk lesions, the majority of cases presented weak to moderate expression, while those at high risk, the fibers were between moderate and strong. When the association between clinical findings and microscopic findings was analyzed, it was possible to observe that smokers with SCC were irregular (P=0.04) and showed a strong expression (P=0.02) when compared to smokers but diagnosed with low or high risk lesion. Our findings suggest possible associations between clinical and microscopic aspects regarding the pattern of collagen type I deposition during the alterations that occur in oral mucosa in the process of malignant transformation.
